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Fig. 8 | BMC Biotechnology

Fig. 8

From: Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Fig. 8

Recognition of SAG1bio-His6 in dependence of MBP as fusion partner. Inlet shows purified MBPtev-SAG1bio-His6 used in this assay. Red bars indicate a non-treated sample, whereas the grey/black bars represent samples that were treated for different time points with TEV protease. Percentages given compare mean MFI values of the respective three treated assays to those of the untreated controls (range in parentheses). P, positive human sera of differing titers; N, negative human sera. The gel image was cropped; the full-length gel is presented in Additional file 8: Supplementary Fig. S2

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