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Fig. 7 | BMC Biotechnology

Fig. 7

From: Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Fig. 7

Discriminatory power between positive and negative sera around the ELIFA cut-off by SAG1bio-His6 and SAG2Abio-His6 employed in the BBMA. A total of 102 sera classified as negative (< 4 IU/mL), equivocal (4 to ≤8 IU/mL) or positive (≥ 8 IU/mL) by automated ELIFA were analyzed by BBMA. a Receiver-operator curve analysis with SAG1bio-His6 as antigen, or b SAG2Abio-His6. Area under curve for SAG1bio-His6: 0.47 for equivocal sera and 0.92 for unequivocal sera. Area under curve for SAG2Abio-His6: 0.58 for equivocal sera and 0.90 for unequivocal sera

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