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Fig. 6 | BMC Biotechnology

Fig. 6

From: Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Fig. 6

Evaluation of anti-SAG1bio-His6 and anti-SAG2Abio-His6 responses by BBMA. a Titration of human sera with different anti-T. gondii titers (in IU) against SAG1bio-His6 (10 ng/1500 Sav-coated beads per sample): Orange - highly positive (> 200 IU/mL), blue - medium positive (63 IU/mL) and gray - negative serum, respectively. b, c Comparison of BBMA MFI of 11 anti-T. gondii antibody-positive and 16 -negative sera with titers determined by a commercial ELISA (Euroimmun) for SAG1bio-His6 (b) and SAG2Abio-His6 (c). Pearson’s correlation coefficients: 0.96 for SAG1bio-His6 and 0.94 for SAG2Abio-His6. d, e Comparison of BBMA MFI of 50 positive and 50 negative sera with titers determined by a commercial ELIFA for SAG1bio-His6 (d) and SAG2Abio-His6 (e). Pearson’s correlation coefficients: 0.96 for SAG1bio-His6 and 0.89 for SAG2Abio-His6. Shaded areas in b-e indicate 95% CI. f Receiver-operator curve comparing the BBMA with the commercial ELIFA for SAG1bio-His6 (gray line) and SAG2Abio-His6 (orange line). Area under curve: 1.0 for SAG1bio-His6 and 0.99 for SAG2Abio-His6

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BMC Biotechnology

ISSN: 1472-6750

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