Fig. 4

Production of soluble fusion-free SAG1 and self-processing of TEV_tev-BirA fusion protein. a Western blot of insoluble (pellet) and soluble fractions (SN) of an induced BioSAG1 lysate with anti-SAG1 mab DG52, indicating substantial soluble and processed protein production of SAG1. b Stained membrane of a bacterial lysate with (TEV-BirA) or without (Ø) plasmid pBAD1030G-TB (left) followed by detection of BirA by a mouse mab directed against it (right). * contamination from left lane. c Silver-stained SDS-PAG of purified SAG1_bio-His₆ (left) containing uncleaved MBP_tev-SAG1_bio-His₆ and detection of biotinylation by Sav-HRPO (right). Images of gels and blots were cropped; full-length blots/gels are presented in Additional file 8: Supplementary Fig. S2