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Fig. 3 | BMC Biotechnology

Fig. 3

From: Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Fig. 3

Expression and purification scheme of SAG1bio(SAG2Abio)-His6. Protein expression of pMJS9 and pBAD1030G-TB is initiated by addition of arabinose (pre-expression), followed after 30 min by rhamnose. The produced MBPtev-SAG1-AviTag-His6 is subsequently biotinylated and the fusion protein is cleaved by TEV. The cleared BioSAG1 (BioSAG2A) lysate is purified by a three-step procedure – affinity chromatography, buffer exchange and removal of MPB-containing proteins

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BMC Biotechnology

ISSN: 1472-6750

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