Table 4 Dialysis buffers used for each Mtb kinase
Kinase | Dialysis buffer |
|---|---|
NDK | 50 mM Tris pH 8.0 |
100 mM KCl | |
1 mM DTT | |
1 mM MgCl2 | |
HSK | 50 mM MOPS pH 8.0 |
150 mM NaCl | |
1 mM DTT | |
10 mM MgCl2 | |
AK and GK | 50 mM Tris pH 7.5 |
150 mM NaCl | |
1 mM DTT | |
5 mM MgCl2 | |
ThiL and RBKS | 50 mM MOPS pH 7.6 |
150 mM NaCl | |
1 mM DTT | |
10 mM MgCl2 | |
AsK | 50 mM Tris pH 6.0 |
200 mM NaCl | |
1 mM DTT | |
10 mM MgCl2 | |
SK | 50 mM Tris pH 7.5 |
1 M NaCl |