Kinase | Gene and Rv identifier | Forward and Reverse primers (5′ → 3′) | Restri-ction Enzyme Recogni-tion site (underli-ned) | Selected pET vector |
---|---|---|---|---|
NDK | ndkA Rv2445c | ndkA-Fwd | pET-16b | |
GGCATATGACCGAACGGACTCTGGTACTG | NdeI | |||
ndkA-Rev | ||||
GTGGATCCTTAGGCGCCGGGAAACCAG | BamHI | |||
HSK | thrB Rv1296 | thrB-Fwd | pET-20a | |
GGCATATGGTGACTCAAGCATTG | NdeI | |||
thrB-Rev | ||||
GTCTCGAGACCGGGAACTCTTACTG | XhoI | |||
AK | ackA Rv0409 | ackA-Fwd | pET-16b | |
GGCATATGGAGTAGCACCGTGCTGGTGATCAA | NdeI | |||
ackA-Rev | ||||
GTGGATCCTTACGCTCGGCGTCCGCCCAG | BamHI | |||
GK | glpK Rv3696c | glpK-Fwd | pET-16b | |
(GGCATATGTCCGACGCCATCCTAG | NdeI | |||
glpK-Rev | ||||
CATGTCGACTTAGGACACGTCAACCCAATCC | SalI | |||
ThiL | thil Rv2977c | thil-Fwd | pET-28a | |
GGTACATATGACCACTAAAGATCACTC | NdeI | |||
thil-Rev | ||||
GATCTCGAGTTACCCTAGCGAACCTTG | XhoI | |||
RBKS | rbks Rv2436 | rbks-Fwd | pET-28a | |
GTACATATGGCAAACGCCAGTGAG | NdeI | |||
rbks-Rev | ||||
GATCTCGAGTTATGAACCGTTGTG | XhoI | |||
AsKa | ask Rv3709c | ask alpha-Fwd | pCDF- | |
GATTACATATGGCGCTCGTCGTGCAG | NdeI | Duet-1 | ||
ask alpha-Rev | (alpha) | |||
GATGTCGACTTACCGTCCCGTCCCCG-3’ | SalI | |||
ask beta-Fwd | pET-26a | |||
GATCATATGGAAGACCCCATCCTGACCG | NdeI | (beta) | ||
ask beta-Rev | ||||
GCCCCTGCCCTGCCCAGCTGTATG | SalI | |||
SK | aroK Rv2539c | Primers were not designed as the aroK plasmid was received from the laboratory of Chris Abell, University of Cambridge | pET-15b |