Table 1 Gene specific primers used for quantitative real-time PCR
Gene | Forward primer (5′- 3′) | Reverese primer (3′ - 5′) | Accession Nr. | Amplicon Size (bp) |
|---|---|---|---|---|
COL2A1 | GCCCAGTTGGGAGTAAGT | CACCAGGATTGCCTTGAA | NM_001844.4 | 106 |
COL1A1 | GCTGGTCCTCCAGGTGAA | GGGGACCAACAGGACCA | NM_000088.3 | 159 |
COL10A1 | TGGCCTGCCTGACTTTA | AATGTCCAGCTCACTGGA | NM_000493.3 | 151 |
ACAN | ACCCAAGGACTGGAATCT | CCTGATCCAGGTAGCCTT | NM_001135.3 | 149 |
COMP | TGCACCGACGTCAACGA | CCGGGTGTTGATGCACA | NM_000095.2 | 231 |
SOX9 | GTGGCTGTAGTAGGAGCT | GCGAACGCACATCAAGA | NM_000346.3 | 155 |
ACTB (reference) | CTGCCCTGAGGCACTC | GTGCCAGGGCAGTGAT | NM_001101.3 | 197 |
RPLP0 (reference) | CAACCCAGCTCTGGAGA | CAGCTGGCACCTTATTGG | BC001834.2 | 116 |
TBP (reference) | CACTTCGTGCCCGAAAC | GCCAGTCTGGACTGTTCT | BC110341.1 | 121 |
POLR2e (reference) | CTATCTGGTGACCCAGGA | CTGCAGAAACTGCTCCA | J04965.1 | 322 |