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Fig. 1 | BMC Biotechnology

Fig. 1

From: Highly enhanced ELISA sensitivity using acetylated chitosan surfaces

Fig. 1

Analysis of His6-c-myc-GST-IL8h recombinant protein obtained in E. coli. In panel a we show 10% acrylamide gel electrophoresis and Coomassie blue staining. Lanes: 1. Molecular weight pattern (Gene On); 2. His6-c-myc-GST-human IL8 (1 μg). The arrow indicates the analyzed protein with the expected MW. In panel b we show the 4PL sigmoidal curve of the ELISA developed using anti-human IL8 or anti-c-myc 9E10 as capture antibody and both revealed using anti- human IL8 biotin. The error bars correspond to three replicates of each concentration of His6-c-myc GST IL8h protein. EC50 represents the analyte concentration capable of generating half of the maximum signal obtained in the test in the linear range of the curve. LOD is the minimum amount of analyte detected and LOQ represents the minimum amount of analyte that can be quantified by the test

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