Table 1 Central Composite Design (CCD) matrix for the experimental and predicted values of wet BC produced by A. senegalensis MA1
From: Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1
Run Order | A: Carbon (Glycerol) | B: Nitrogen (Yeast extract) | C: pH | D: Temperature | E: Additives (PEG 6000) | Actual wet weight of BC | Predicted wet weight of BC |
|---|---|---|---|---|---|---|---|
|  | (mL L− 1) | (g L− 1) |  | (°C) | (g L− 1) | (g L− 1) | (g L− 1) |
1 | 50.00 | 7.50 | 6.00 | 33.50 | 1.76 | 387.83 | 343.13 |
2 | 55.00 | 5.00 | 8.00 | 37.00 | 1.00 | 0.00 | 67.06 |
3 | 55.00 | 10.00 | 8.00 | 37.00 | 3.00 | 0.00 | 19.31 |
4 | 45.00 | 10.00 | 4.00 | 30.00 | 3.00 | 0.00 | 244.17 |
5 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 440.46 | 378.60 |
6 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 418.89 | 378.60 |
7 | 61.89 | 7.50 | 6.00 | 33.50 | 3.00 | 370.50 | 185.46 |
8 | 45.00 | 10.00 | 4.00 | 37.00 | 1.00 | 0.00 | −53.09 |
9 | 45.00 | 5.00 | 4.00 | 37.00 | 1.00 | 23.80 | 28.08 |
10 | 55.00 | 10.00 | 8.00 | 30.00 | 1.00 | 22.43 | 148.3 |
11 | 55.00 | 5.00 | 4.00 | 30.00 | 1.00 | 374.51 | 507.71 |
12 | 55.00 | 10.00 | 4.00 | 37.00 | 1.00 | 0.00 | 92.73 |
13 | 55.00 | 10.00 | 4.00 | 37.00 | 3.00 | 0.00 | 80.39 |
14 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 394.57 | 378.60 |
15 | 50.00 | 7.50 | 1.24 | 33.50 | 1.00 | 0.00 | −157.43 |
16 | 45.00 | 10.00 | 4.00 | 37.00 | 1.00 | 0.00 | 41.20 |
17 | 55.00 | 5.00 | 4.00 | 37.00 | 5.00 | 0.00 | 102.59 |
18 | 45.00 | 5.00 | 4.00 | 37.00 | 1.00 | 0.00 | 8.60 |
19 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 436.37 | 378.60 |
20 | 45.00 | 10.00 | 8.00 | 37.00 | 1.00 | 0.00 | 76.91 |
21 | 50.0 | 7.50 | 6.00 | 25.18 | 3.00 | 430.41 | 361.36 |
22 | 45.00 | 5.00 | 4.00 | 37.00 | 1.00 | 0.00 | 8.60 |
23 | 55.00 | 10.00 | 4.00 | 37.00 | 5.00 | 0.00 | 45.14 |
24 | 45.00 | 5.00 | 4.00 | 30.00 | 5.00 | 415.10 | 265.81 |
25 | 45.00 | 10.00 | 6.00 | 37.00 | 5.00 | 388.63 | 450.91 |
26 | 55.00 | 5.00 | 8.00 | 30.00 | 5.00 | 0.00 | 182.28 |
27 | 45.00 | 5.00 | 4.00 | 30.00 | 3.00 | 0.00 | 248.71 |
28 | 45.00 | 10.00 | 4.00 | 30.00 | 1.00 | 334.97 | 92.48 |
29 | 50.00 | 7.50 | 6.00 | 41.82 | 3.00 | 0.00 | −118.12 |
30 | 50.00 | 7.50 | 6.00 | 33.50 | 7.76 | 469.83 | 432.62 |
31 | 50.00 | 13.45 | 6.00 | 33.50 | 3.00 | 456.57 | 328.06 |
32 | 45.00 | 5.00 | 4.00 | 37.00 | 1.76 | 0.00 | 28.08 |
33 | 45.00 | 5.00 | 8.00 | 30.00 | 1.00 | 363.41 | 320.58 |
34 | 55.00 | 5.00 | 4.00 | 37.00 | 1.00 | 386.85 | 373.54 |
35 | 55.00 | 10.00 | 4.00 | 30.00 | 1.00 | 383.61 | 234.12 |
36 | 45.00 | 10.00 | 4.00 | 30.00 | 5.00 | 396.92 | 372.95 |
37 | 55.00 | 5.00 | 4.00 | 30.00 | 5.00 | 293.88 | 228.08 |
38 | 55.00 | 5.00 | 8.00 | 30.00 | 1.00 | 383.89 | 271.63 |
39 | 55.00 | 5.00 | 8.00 | 37.00 | 3.00 | 0.00 | 38.18 |
40 | 50.00 | 7.50 | 10.76 | 33.50 | 3.00 | 0.00 | −18.73 |
41 | 45.00 | 5.00 | 8.00 | 37.00 | 1.00 | 97.29 | 105.56 |
42 | 55.00 | 10.00 | 4.00 | 30.00 | 1.00 | 0.00 | 230.50 |
43 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 378.42 | 378.60 |
44 | 55.00 | 10.00 | 8.00 | 37.00 | 1.76 | 0.00 | −29.4 |
45 | 50.00 | 1.55 | 6.00 | 33.50 | 3.00 | 414.57 | 355.91 |
46 | 38.11 | 7.50 | 6.00 | 33.50 | 3.00 | 399.43 | 397.29 |
47 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 408.08 | 378.68 |
48 | 45.00 | 10.00 | 4.00 | 30.00 | 1.00 | 0.00 | 92.48 |
49 | 50.00 | 7.50 | 6.00 | 33.50 | 1.00 | 297.98 | 343.13 |
50 | 50.00 | 7.50 | 6.00 | 33.50 | 3.00 | 457.81 | 378.60 |