TY - JOUR AU - Tanihara, Fuminori AU - Hirata, Maki AU - Nguyen, Nhien Thi AU - Sawamoto, Osamu AU - Kikuchi, Takeshi AU - Doi, Masako AU - Otoi, Takeshige PY - 2020 DA - 2020/08/18 TI - Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes JO - BMC Biotechnology SP - 40 VL - 20 IS - 1 AB - Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galactose, the major xenoantigen causing hyperacute rejection. GGTA1-modified pigs, therefore, are promising donors for pig-to-human xenotransplantation. In this study, we developed a method for the introduction of the CRISPR/Cas9 system into in vitro-fertilized porcine zygotes via electroporation to generate GGTA1-modified pigs. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-020-00638-7 DO - 10.1186/s12896-020-00638-7 ID - Tanihara2020 ER -