Fig. 3
From: Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome
Monolayer formation assay. a Images of bright and GFP fluorescence from cells seeded on the membrane of a Millicell-24 plate. In Caco-2 4CYPs-MAC #1, cells did not spread throughout the membrane and did not form a cell layer, but in Caco-2 4CYPs-MAC #2, there were no gaps between cells, and they formed a cell layer. The white bars indicate a distance of 100 μm. b Transepithelial electrical resistance (TEER) values of Caco-2 cells, Caco-2 4CYPs-MAC #1 and Caco-2 4CYPs-MAC #2 cells. c Culture time-dependent change in gene expression. The relative expression level was evaluated in Caco-2 and Caco-2 4CYPs-MAC #2 (mean ± S.E., n = 3)