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Fig. 1 | BMC Biotechnology

Fig. 1

From: Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome

Fig. 1

Introduction of the 4CYPs-MAC into Caco-2 cells. a Transfer of the 4CYPs-MAC into Caco-2 cells. The structure of the MAC carrying four CYPs and POR is shown at the top. A CAG promoter was placed upstream of each gene. A schematic view of the transfer of the 4CYPs-MAC to Caco-2 cells is shown at the bottom. The 4CYPs-MAC was transferred from CHO cells to Caco-2 cells through the MMCT method. b An image of GFP fluorescence in parental Caco-2 cells and Caco-2 4CYPs-MAC cells. The GFP fluorescence indicates the presence of the 4CYPs-MAC in the Caco-2 cells. The white bars indicate a distance of 50 μm. c Results of genomic PCR analyses to detect four CYP and POR transgenes on the MAC in Caco-2 cells. Donor CHO cells and Caco-2 cells are positive and negative controls, respectively. d Images of FISH analyses of Caco-2 cells carrying the 4CYPs-MAC. Red and green signals indicate the MAC and transgenes, respectively. The arrow shows the 4CYPs-MAC and the inset shows an enlarged image of the 4CYPs-MAC

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