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Table 1 Stepwise CPA introduction and withdrawal

From: Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

CPA addition steps CPA washout steps
Equilibration solution
10 min
CPA (VM3)
1 min
0.5 M sucrose
5 min
0.25 M sucrose
5 min
0 M sucrose
5 min