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Table 1 Stepwise CPA introduction and withdrawal

From: Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

CPA addition steps

CPA washout steps

Equilibration solution

10 min

CPA (VM3)

1 min

0.5 M sucrose

5 min

0.25 M sucrose

5 min

0 M sucrose

5 min