Fig. 3

Agroinjection-mediated transient expression in tomato fruit. Results from an Agrobacterium-mediated transient expression assay testing the functionality of the candidate fruit-specific promoters in unripe (left) and ripe (right) tomato fruits is shown. Control, wildtype tomato fruit infiltrated with an empty vector is shown on the top, followed below by those infiltrated with the promoter construct as labeled. Each fruit was histochemically stained for β-glucuronidase activity 4 days after agroinjection. The CitVO2p construct is a CitVO1 promoter fragment lacking the native intron. Scale bar – 1 cm