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Fig. 6 | BMC Biotechnology

Fig. 6

From: Correction to: CRISPR-Cas9 mediated targeted disruption of FAD2–2 microsomal omega-6 desaturase in soybean (Glycine max.L)

Fig. 6

Agrobacterium mediated stable transformation of soybean. From A to F showed the series of different media to acquired stable transgenic soybean plants. a Sterilized soybean seeds were germinated on germination medium. b After infection with agrobacterium the seeds were planted in co-culture medium under controlled environment. c Collection of transgenic plants on a selective medium comprising 6 mg l−1glufosinate where left side medium indicates sensitivity while right side showed resistance to herbicide (glufosinate). d Glufosinate resistant seedlings were incubated on shoot elongation medium after 2 weeks. e Establishment of rootswere observed after 2 weeks of incubation on rooting medium. f A full flourish plant was shifted to sterile soil in a growth chamber

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