TY - JOUR AU - Gruteser, Nadine AU - Kohlhas, Viktoria AU - Balfanz, Sabine AU - Franzen, Arne AU - Günther, Anne AU - Offenhäusser, Andreas AU - Müller, Frank AU - Nikolaev, Viacheslav AU - Lohse, Martin J. AU - Baumann, Arnd PY - 2020 DA - 2020/08/27 TI - Establishing a sensitive fluorescence-based quantification method for cyclic nucleotides JO - BMC Biotechnology SP - 47 VL - 20 IS - 1 AB - Approximately 40% of prescribed drugs exert their activity via GTP-binding protein-coupled receptors (GPCRs). Once activated, these receptors cause transient changes in the concentration of second messengers, e.g., cyclic adenosine 3′,5′-monophosphate (cAMP). Specific and efficacious genetically encoded biosensors have been developed to monitor cAMP fluctuations with high spatial and temporal resolution in living cells or tissue. A well characterized biosensor for cAMP is the Förster resonance energy transfer (FRET)-based Epac1-camps protein. Pharmacological characterization of newly developed ligands acting at GPCRs often includes numerical quantification of the second messenger amount that was produced. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-020-00633-y DO - 10.1186/s12896-020-00633-y ID - Gruteser2020 ER -