Table 1 Solubilization of NM spidroins from IBs with different concentrations of urea through two different methods
Urea conc. (mol/L) | Protein concentration (ng/ml) | |
|---|---|---|
One-step heating method | Traditional urea-denatured | |
0 | 483 | 0 |
1 | 952 | 58 |
2 | 1290 | 157 |
3 | 1866 | 311 |
4 | 2335 | 728 |
5 | 2641 | 1368 |
6 | 2786 | 2143 |
7 | 2912 | 2458 |