Table 1 Reference organisms used in this study and analytical specificity evaluation results
Strains | Serogroup or species | No. of isolates | Analytical specificity of primers | |||
|---|---|---|---|---|---|---|
PCR | nPCR | qPCR | LAMP | |||
Entamoeba spp. | Entamoeba histolytica HM-1:IMSS | 1 | + | + | + | + |
Entamoeba dispar SAW760 | 1 | – | – | – | – | |
Entamoeba moshkovskii Laredo | 1 | – | – | – | – | |
Other enteric pathogens (n = 75) | Acinetobacter baumannii | 1 | – | – | – | – |
Aeromonas hydrophila | 2 | – | – | – | – | |
Bacillus subtilis | 1 | – | – | – | – | |
Burkholderia cepacia | 1 | – | – | – | – | |
Burkholderia pseudomallei | 1 | – | – | – | – | |
Burkholderia thailandensis | 1 | – | – | – | – | |
Citrobacter freundii | 1 | – | – | – | – | |
Enterococcus faecalis | 1 | – | – | – | – | |
Enterococcus faecium | 1 | – | – | – | – | |
Enterococcus gallinarum | 1 | – | – | – | – | |
Enterohemorrhagic Escherichia coli | 1 | – | – | – | – | |
Enteroinvasive Escherichia coli | 1 | – | – | – | – | |
Enteropathogenic Escherichia coli | 1 | – | – | – | – | |
Enterotoxigenic Escherichia coli | 1 | – | – | – | – | |
Escherichia coli | 1 | – | – | – | – | |
group A Streptococcus | 1 | – | – | – | – | |
group B Streptococcus | 1 | – | – | – | – | |
group F Streptococcus | 1 | – | – | – | – | |
group G Streptococcus | 1 | – | – | – | – | |
Haemophilus influenza | 1 | – | – | – | – | |
Klebsiella pneumoniae | 2 | – | – | – | – | |
Leptospira biflexa ser. Patoc | 1 | – | – | – | – | |
Leptospira interrogans ser. Canicola | 1 | – | – | – | – | |
Leptospira interrogans ser. Hebdomadis | 1 | – | – | – | – | |
Leptospira interrogans ser. Pomona | 1 | – | – | – | – | |
Leptospira licerasiae ser. Varillal | 1 | – | – | – | – | |
Listeria monocytogenes | 1 | – | – | – | – | |
Salmonella enterica ser. Agona | 1 | – | – | – | – | |
Salmonella enterica ser. Albany | 1 | – | – | – | – | |
Salmonella enterica ser. Bardo | 1 | – | – | – | – | |
Salmonella enterica ser. Bordeaux | 1 | – | – | – | – | |
Salmonella enterica ser. Braenderup | 1 | – | – | – | – | |
Salmonella enterica ser. Emek | 1 | – | – | – | – | |
Salmonella enterica ser. Enteritidis | 1 | – | – | – | – | |
Salmonella enterica ser. Hadar | 1 | – | – | – | – | |
Salmonella enterica ser. Heidelberg | 1 | – | – | – | – | |
Salmonella enterica ser. Java | 1 | – | – | – | – | |
Salmonella enterica ser. Kibi | 1 | – | – | – | – | |
Salmonella enterica ser. Kissi | 1 | – | – | – | – | |
Salmonella enterica ser. Newport | 1 | – | – | – | – | |
Salmonella enterica ser. Oslo | 1 | – | – | – | – | |
Salmonella enterica ser. Paratyphi A | 2 | – | – | – | – | |
Salmonella enterica ser. Paratyphi B | 2 | – | – | – | – | |
Salmonella enterica ser. Paratyphi C | 1 | – | – | – | – | |
Salmonella enterica ser. Poona | 1 | – | – | – | – | |
Salmonella enterica ser. Regent | 1 | – | – | – | – | |
Salmonella enterica ser. Richmond | 1 | – | – | – | – | |
Salmonella enterica ser. Tshiongwe | 1 | – | – | – | – | |
Salmonella enterica ser. Typhi | 2 | – | – | – | – | |
Salmonella enterica ser. Typhimurium | 1 | – | – | – | – | |
Salmonella enterica ser. Uppsala | 1 | – | – | – | – | |
Salmonella enterica ser. Virchow | 1 | – | – | – | – | |
Salmonella enterica ser. Weltevreden | 1 | – | – | – | – | |
Shigella boydii | 2 | – | – | – | – | |
Shigella dysenteriae | 2 | – | – | – | – | |
Shigella flexneri | 2 | – | – | – | – | |
Shigella sonnei | 2 | – | – | – | – | |
Staphylococcus aureus | 1 | – | – | – | – | |
Staphylococcus epidermidis | 1 | – | – | – | – | |
Vibrio cincinnatiensis | 1 | – | – | – | – | |
Vibrio fluvialis | 1 | – | – | – | – | |
Vibrio mimicus | 1 | – | – | – | – | |
Vibrio parahaemolyticus | 1 | – | – | – | – | |
Vibrio vulnificus | 1 | – | – | – | – | |
Yersinia enterocolitica | 2 | – | – | – | – | |
Total | Â | 78 | Â | Â | Â | Â |