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Table 2 Analysis of large scale purification runs

From: Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance for virus purification

  Total % of Total
Infectious Particles (TCID50)b Protein (ug) DNA (ug) Infectious Particles (TCID50)b Protein (ug) DNA (ug)
Run #1
Feed 1.67E+ 10 404.9 22.95 100% 100% 100%
Flow Through 2.04E+ 09 164.4 14.11 12.2% 64% 62%
Wash 1 3.36E+ 08 0a 0.50 2.0% 0.0% 2.2%
Wash 2 8.00E+ 06 0a 0a 0.05% 0.0% 0a
Elution 1 (20 mL) 1.05E+ 10 19.1 8.95 63 4.7% 39%
Elution 2 (20 mL) 9.38E+ 08 18.6 0.69 6% 4.6% 3%
Run #2
Feed 5.58E+ 09 504.3 21.16 100% 100% 100%
Flow Through 3.14E+ 08 349.7 12.73 6% 69% 60.17%
Wash 1 1.25E+ 07 25.55 0.37 0.22% 5% 1.77%
Wash 2 1.91E+ 06 21.1 0a 0.03% 4.2% 0.00%
Elution 1 (20 mL) 3.56E+ 09 12.7 8.43 64% 2.5% 39.83%
Elution 2 (20 mL) 2.42E+ 08 10.1 0.35 4% 2.0% 1.66%
Run #3
Feed 4.84E+ 09 610.7 17.028 100% 100% 100%
Flow Through 9.14E+ 08 482.3 15.114 18.88% 75.91% 88.8%
Wash 1 2.10E+ 08 46.7 0.534 4.34% 7.65% 3.1%
Wash 2 1.19E+ 06 36.6 0a 0.02% 6.00% 0a%
Elution 1 (20 mL) 3.61E+ 09 22.2 1.91 74.64% 3.64% 11.2%
Elution 2 (20 mL) 1.21E+ 08 24.3 0.237 2.49% 3.98% 1.4%
  1. aBelow level of detection
  2. bNumber of infectious particles were determined in triplicate using the TCID50 method