Table 2 Analysis of large scale purification runs
| Â | Total | % of Total | ||||
|---|---|---|---|---|---|---|
Infectious Particles (TCID50)b | Protein (ug) | DNA (ug) | Infectious Particles (TCID50)b | Protein (ug) | DNA (ug) | |
Run #1 | ||||||
Feed | 1.67E+ 10 | 404.9 | 22.95 | 100% | 100% | 100% |
Flow Through | 2.04E+ 09 | 164.4 | 14.11 | 12.2% | 64% | 62% |
Wash 1 | 3.36E+ 08 | 0a | 0.50 | 2.0% | 0.0% | 2.2% |
Wash 2 | 8.00E+ 06 | 0a | 0a | 0.05% | 0.0% | 0a |
Elution 1 (20 mL) | 1.05E+ 10 | 19.1 | 8.95 | 63 | 4.7% | 39% |
Elution 2 (20 mL) | 9.38E+ 08 | 18.6 | 0.69 | 6% | 4.6% | 3% |
Run #2 | ||||||
Feed | 5.58E+ 09 | 504.3 | 21.16 | 100% | 100% | 100% |
Flow Through | 3.14E+ 08 | 349.7 | 12.73 | 6% | 69% | 60.17% |
Wash 1 | 1.25E+ 07 | 25.55 | 0.37 | 0.22% | 5% | 1.77% |
Wash 2 | 1.91E+ 06 | 21.1 | 0a | 0.03% | 4.2% | 0.00% |
Elution 1 (20 mL) | 3.56E+ 09 | 12.7 | 8.43 | 64% | 2.5% | 39.83% |
Elution 2 (20 mL) | 2.42E+ 08 | 10.1 | 0.35 | 4% | 2.0% | 1.66% |
Run #3 | ||||||
Feed | 4.84E+ 09 | 610.7 | 17.028 | 100% | 100% | 100% |
Flow Through | 9.14E+ 08 | 482.3 | 15.114 | 18.88% | 75.91% | 88.8% |
Wash 1 | 2.10E+ 08 | 46.7 | 0.534 | 4.34% | 7.65% | 3.1% |
Wash 2 | 1.19E+ 06 | 36.6 | 0a | 0.02% | 6.00% | 0a% |
Elution 1 (20 mL) | 3.61E+ 09 | 22.2 | 1.91 | 74.64% | 3.64% | 11.2% |
Elution 2 (20 mL) | 1.21E+ 08 | 24.3 | 0.237 | 2.49% | 3.98% | 1.4% |