| |
Total
|
% of Total
|
|---|
|
Infectious Particles (TCID50)b
|
Protein (ug)
|
DNA (ug)
|
Infectious Particles (TCID50)b
|
Protein (ug)
|
DNA (ug)
|
|---|
|
Run #1
|
|
Feed
|
1.67E+ 10
|
404.9
|
22.95
|
100%
|
100%
|
100%
|
|
Flow Through
|
2.04E+ 09
|
164.4
|
14.11
|
12.2%
|
64%
|
62%
|
|
Wash 1
|
3.36E+ 08
|
0a
|
0.50
|
2.0%
|
0.0%
|
2.2%
|
|
Wash 2
|
8.00E+ 06
|
0a
|
0a
|
0.05%
|
0.0%
|
0a
|
|
Elution 1 (20 mL)
|
1.05E+ 10
|
19.1
|
8.95
|
63
|
4.7%
|
39%
|
|
Elution 2 (20 mL)
|
9.38E+ 08
|
18.6
|
0.69
|
6%
|
4.6%
|
3%
|
|
Run #2
|
|
Feed
|
5.58E+ 09
|
504.3
|
21.16
|
100%
|
100%
|
100%
|
|
Flow Through
|
3.14E+ 08
|
349.7
|
12.73
|
6%
|
69%
|
60.17%
|
|
Wash 1
|
1.25E+ 07
|
25.55
|
0.37
|
0.22%
|
5%
|
1.77%
|
|
Wash 2
|
1.91E+ 06
|
21.1
|
0a
|
0.03%
|
4.2%
|
0.00%
|
|
Elution 1 (20 mL)
|
3.56E+ 09
|
12.7
|
8.43
|
64%
|
2.5%
|
39.83%
|
|
Elution 2 (20 mL)
|
2.42E+ 08
|
10.1
|
0.35
|
4%
|
2.0%
|
1.66%
|
|
Run #3
|
|
Feed
|
4.84E+ 09
|
610.7
|
17.028
|
100%
|
100%
|
100%
|
|
Flow Through
|
9.14E+ 08
|
482.3
|
15.114
|
18.88%
|
75.91%
|
88.8%
|
|
Wash 1
|
2.10E+ 08
|
46.7
|
0.534
|
4.34%
|
7.65%
|
3.1%
|
|
Wash 2
|
1.19E+ 06
|
36.6
|
0a
|
0.02%
|
6.00%
|
0a%
|
|
Elution 1 (20 mL)
|
3.61E+ 09
|
22.2
|
1.91
|
74.64%
|
3.64%
|
11.2%
|
|
Elution 2 (20 mL)
|
1.21E+ 08
|
24.3
|
0.237
|
2.49%
|
3.98%
|
1.4%
|
- aBelow level of detection
- bNumber of infectious particles were determined in triplicate using the TCID50 method
