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Table 1 Ions formed at pH 8 for several interference agents (bicarbonate, citrate, EDTA, and phosphate)

From: Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance for virus purification

Buffer Species

pKa

Ion Form at pH 8

Bicarbonate

6.40, 10.30

1−

Phosphate

2.10, 7.20, 12.30

2−

EDTA

2.00, 2.70, 6.20, 10.31

3−

Citrate

3.13, 4.76, 6.40

3−

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BMC Biotechnology

ISSN: 1472-6750

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