Skip to main content
Fig. 5 | BMC Biotechnology

Fig. 5

From: Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance for virus purification

Fig. 5

Capacity test with optimal interference condition of 100 mM citrate buffer. Under the interference condition of 100 mM citrate, the sample load was increased to determine the NDV loading capacity. NDV containing allantoic fluid (4.22 × 107 TCID50/mL) was loaded onto the column (VL). The membrane reached capacity at approximately 170 membrane volumes (MV) (*), where an increase in virus was observed in the flow through. Breakthrough continued until approximately 350 MV (+), where the virus in the flow through was similar to the concentration of input virus. At this point, a wash step (W) was applied to the membrane, followed by an elution step (E) at 460 MV. The reconstructed chromatogram was created from offline measurements of NDV titres from fractions taken throughout the experiment

Back to article page