TY - JOUR AU - Gratacap, Remi L. AU - Regan, Tim AU - Dehler, Carola E. AU - Martin, Samuel A. M. AU - Boudinot, Pierre AU - Collet, Bertrand AU - Houston, Ross D. PY - 2020 DA - 2020/06/23 TI - Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system JO - BMC Biotechnology SP - 35 VL - 20 IS - 1 AB - Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation. Salmonids are the most important aquaculture species by value, and improving genetic resistance to infectious disease is a major goal. However, use of genome editing to evaluate putative disease resistance genes in cell lines, and the use of genome-wide CRISPR screens is currently limited by a lack of available tools and techniques. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-020-00626-x DO - 10.1186/s12896-020-00626-x ID - Gratacap2020 ER -