Table 6 The primers and probe sequences for the virulence factors
Virulence Factor | Category | Sequence (5′ → 3′)a |
|---|---|---|
vacA s1 | Forward primer (F) | ACACCGCAAAATCAATCGCC |
Reverse primer (R) | CCCCAACAATGGCTGGAATG | |
Probe 1 (P1) | HEX-GCATCACACCGCAACAAAGT-BHQ2 | |
Probe 2 (P2) | HEX-GCGCCATACCGCAAGAGAGT-BHQ2 | |
vacA m1 | Forward primer 1 (F1) | ATCAATTATTTGGTCCGAGGC |
Forward primer 2 (F2) | ATCAACTATCTGGTCCGAGGC | |
Reverse primer (R) | GCTGTTAATCTTCATGAGCGGT | |
Probe 1 (P1) | FAM-GATAGCGCGACTGGGTTTTA-BHQ1 | |
Probe 2 (P2) | FAM-GACAGCGCGACCGGATTTTA-BHQ1 | |
cagA | Forward primer (F) | GTGCCTGCTAGTTTGTCAGC |
Reverse primer (R) | ACGAGCTTAAGCCACTCAGG | |
Probe 1 (P) | Texas Red-GCTATTAACAGCCACACACGC-Dabcyl | |
babA2 | Forward primer (F) | GAAATCCCTAATACTAAATCC |
Reverse primer (R) | GTAAAAGCCGTCGTCTTCAGC | |
Probe 1 (P1) | CY5-GGAGAAAAAACATGAAAAAACA-BHQ3 | |
Probe 2 (P2) | CY5-GGGGAGAAAACATGAAAAAACA-BHQ3 |