Fig. 4

CD19CAR T cells demonstrate killing of Caco-2 CD19+ cysts. a Representative confocal micrographs showing specific killing of Caco-2 GFP+/CD19+ cysts mediated by CD19CAR T cells. Cells were marked with Hoechst 33242 and actin. Scale bar represents 10 μm. Yellow arrows outline the presence of T-cells. b BLI killing assay of Caco-2 cysts expressing CD19 or not, co-cultured with CD19CAR or Mock T cells. Data represent mean ± S.D. of hexaplicates. Representative data from one of three experiments are shown. Statistics analysis were conducted from timepoints 3 to 7 (2-way ANOVA). c Total apoptosis signal as measured by Annexin V of Caco-2 cysts expressing CD19 or not, co-cultured with CD19CAR or Mock T cells and imaged by high throughput microscope. Data represent mean ± S.D. of octuplicates. Representative data from one of three experiments are shown. Statistics analysis were conducted at timepoint 232 (1-way ANOVA). d Corresponding time lapse of the killing assay described in C. Scale bars represent 80 μm. Yellow arrows outline the presence of T-cells