Fig. 3

CD19 is expressed on the surface of Caco-2 cells and do not hinder their ability to form cysts. a BLI killing assay of Caco-2 cells expressing CD19 or not, co-cultured with CD19CAR or Mock T cells (E:T ratio of 1:10). Data represent mean ± S.D. of hexaplicates. Representative data from one of three experiments are shown. Statistics analysis were conducted from timepoints 3 to 7 (2-way ANOVA). b Time lapse of Caco-2 GFP+/CD19+ cysts formation observed with a high-throughput microscope. Scale bar represents 20 μm. c Airyscan micrographs showing the establishment of a basal/apical polarity on a Caco-2 organoid marked by Hoechst 33342, PKC-ζ, E-cadherin and actin. Scale bar represents 10 μm