Fig. 4From: Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free systemCinnamyl acetate synthesis through transesterification in solvent-free system. (a) reaction equation of cinnamyl acetate synthesis. (b)(c) Effect of substrate concentration (0.1 M, 0.3 M, 0.5 M, 0.8 M, 1.0 M) and reaction time (lanes 1–4, reaction time 1–4 h) (TLC analysis). (d) Effect of addition of water (1, 2, 4, 5%) (TLC analysis). (e) Effect of addition of enzyme (TLC). (f) HPLC analysis of substrate and productsBack to article page