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Fig. 2 | BMC Biotechnology

Fig. 2

From: Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system

Fig. 2

SDS-PAGE analysis and enzymatic properties of EstGSU753 expressed in E. coli. (a) SDS-PAGE analysis of EstGSU753 expressed in E. coli. (IPTG concentration: 0.1 mM, induction time: 20 h). Lane M: protein marker; lanes 1–3: cell lysate, supernatant and purification of EstGSU753. (b) Determination of the optimum substrate for recombinant EstBASΔSP. pNP-acetate (C2), pNP-butyrate (C4), pNP-hexanoate (C6), pNP-caprylate (C8), pNP-decanoate (C10), pNP-laurate (C12), pNP-myristate (C14) and pNP-palmitate (C16) (c) Effect of temperature on the activities (pH 8.0). (d) Effect of pH on the activities (60 °C)

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