Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

BMC Biotechnology

Table 2 Efficiency of PROGED::gPDS mutagenesis with Km selection for 3 days

Transformed organ	Counter-selection	Strain	No. of groups with mutants^a	Number of mutants	Efficiency (%)^b
Tuber		gv2260	1	1	2
		LBA4404	2	3	7
	5-FC	gv2260	3	3	9
	5-FC	LBA4404	2	2	5
Leaf		gv2260	2	3	9
		LBA4404	3	4	10
	5-FC	gv2260	2	2	5
	5-FC	LBA4404	4	4	10

^a Seven groups of regenerated plants were tested from each transformation experiment. Each group consisted of six plants
^b Efficiency (%) of PDS mutagenesis was calculated as number of mutants detected by the restriction enzyme site loss method in PCR amplification of genomic DNA after digestion with MlyI / number of tested plants (7 groups × 6 plants = 42) × 100

ISSN: 1472-6750