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Table 2 Efficiency of PROGED::gPDS mutagenesis with Km selection for 3 days

From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Transformed organCounter-selectionStrainNo. of groups with mutantsaNumber of mutantsEfficiency (%)b
Tuber gv2260112
LBA4404237
5-FCgv2260339
LBA4404225
Leaf gv2260239
LBA44043410
5-FCgv2260225
LBA44044410
  1. a Seven groups of regenerated plants were tested from each transformation experiment. Each group consisted of six plants
  2. b Efficiency (%) of PDS mutagenesis was calculated as number of mutants detected by the restriction enzyme site loss method in PCR amplification of genomic DNA after digestion with MlyI / number of tested plants (7 groups × 6 plants = 42) × 100