Fig. 1
From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation
Schematic drawing of the PROGED vector with the gPDS coding sequence and the adjacent PAM motive that is essential for cleavage by Cas9 nuclease. Plant promoter regions are red-coloured. RB, right border of T-DNA; nptIII, neomycin phosphotransferase III with a bacterial promoter providing Km resistance for bacteria; codA-nptII encodes the fusion protein suitable for positive/negative (Km/5-FC) selection of gene-edited mutants after plant transformation