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Fig. 1 | BMC Biotechnology

Fig. 1

From: Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Fig. 1

Schematic drawing of the PROGED vector with the gPDS coding sequence and the adjacent PAM motive that is essential for cleavage by Cas9 nuclease. Plant promoter regions are red-coloured. RB, right border of T-DNA; nptIII, neomycin phosphotransferase III with a bacterial promoter providing Km resistance for bacteria; codA-nptII encodes the fusion protein suitable for positive/negative (Km/5-FC) selection of gene-edited mutants after plant transformation

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