Fig. 2

Cloning and production of 10 proteins from a single baculovirus. a. XhoI digestion of a plasmid containing expression cassettes for the 7 proteins of the proteosomal lid complex plus Hsp40, 70, and 90. The pattern of bands expected for the DNA sizing ladder and the XhoI digest are shown on the left. The actual ladder and digest samples were separated and analyzed via agarose gel electrophoresis and are shown on the right. b. A baculovirus generated from the clone shown in A was used to test whether the proteins from all 10 encoded genes are recombinantly expressed. Western blot analysis of total insect cell lysate using antibodies against epitope tags present on each protein indicates that all 10 proteins are expressed from the single baculovirus