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Table 2 Plasmids used in this study

From: Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli

NameKey featuresSource
pZS4Int-tetRGene encoding the TetR repressor under control of the constitutive PN25 promoter, SC101 ori, Spr[45]
pMA7CR_2.0λ Red β-protein under control of the L-arabinose inducible PBAD, Cas9 under control of the anhydrotetracycline inducible PLtet, ColE1 ori, Ampr[30]
pMAZ-SKsgRNA targeting galK under control of the anhydrotetracycline inducible PLtet, L-rhamnose inducible plasmid self-killing mechanism, ColA ori, Kmr[30]
pMAZ-SK_xpMAZ-SK with gRNA variants targeting x gene:
dsbA
dsbB
skp
sppA
degP
This study
pFREECas9 under control of the anhydrotetracycline inducible PLtet, gRNA targeting the most common replicons under control of the L-rhamnose inducible Prha, ColA ori, tetR, Kmr[31]
pSB-M1spelB-scFv173–2-5-phoA fusion gene under control of the m-toluate inducible XylS/Pm promoter system, RK2 replicon, Kmr[25]
pGM29ompAgene encoding GM-CSF with ompA signal sequence under control of the m-toluate inducible XylS/Pm promoter system, RK2 replicon, Ampr[26]