Table 1 In silico analysis of TIR values and RBS sequences (denoted in capital letters) with adjacent genomic regions
Gene | Native TIR (au) | Regulated TIR (au) | Native RBS (5′ – 3′) | Synthetic RBS (5′ – 3′) a |
|---|---|---|---|---|
dsbA | 38,952 | 387,845 | //−tgtaTTAATCGGAGAGAGTAGATCatga−// | //−gatcAACTAAGGAGGCTTATTatga−// |
dsbB | 158 | 1783 | //−tatgCATATTGCAGGGAAATGATTatgt−// | //−gattGCGCACACCGAAGTTGGAATACGATTGatgt−// |
skp | 19,377 | 80,140 | //−ccggTGCAAATGGGATGGTAAGGAGTTTATTgtga−// | //−ccggCAGTATATACGTCTAACACTTAGGGGGAATATTAtga−// |
sppA | 4205 | 19,838 | //−tattGCGCCTGTGACAGGTGTGACCTTAAGTTGGGAGAATACatgc−// | //−tattCAAACGTACCCCTTAATTATACCTAACGAGGAGAAACTatgc−// |
degP | 2139 | 286 | //−ttttGCGTTATCTGTTAATCGAGACTGAAATACatga−// | //−ttttGAGTAACGCCACTGATCGAAATTGAGGAAGtga−// |