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Table 1 In silico analysis of TIR values and RBS sequences (denoted in capital letters) with adjacent genomic regions

From: Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli

GeneNative TIR (au)Regulated TIR (au)Native RBS (5′ – 3′)Synthetic RBS (5′ – 3′) a
dsbA38,952387,845//−tgtaTTAATCGGAGAGAGTAGATCatga−////−gatcAACTAAGGAGGCTTATTatga−//
dsbB1581783//−tatgCATATTGCAGGGAAATGATTatgt−////−gattGCGCACACCGAAGTTGGAATACGATTGatgt−//
skp19,37780,140//−ccggTGCAAATGGGATGGTAAGGAGTTTATTgtga−////−ccggCAGTATATACGTCTAACACTTAGGGGGAATATTAtga−//
sppA420519,838//−tattGCGCCTGTGACAGGTGTGACCTTAAGTTGGGAGAATACatgc−////−tattCAAACGTACCCCTTAATTATACCTAACGAGGAGAAACTatgc−//
degP2139286//−ttttGCGTTATCTGTTAATCGAGACTGAAATACatga−////−ttttGAGTAACGCCACTGATCGAAATTGAGGAAGtga−//
  1. a Desired TIR values for skp and degP were obtained by RBS replacement and substitution of the first base (underlined above) of a start codon; GTG → ATG and ATG → GTG, respectively