Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation

Fig. 1

A The schematic maps of recombinant pAMJ1653 vector (R-pAMJ1653) harboring cLFchimera and schematic representation of cLFchimera and its parts. P170 promoter, L. lactis promoter is up-regulated by low-pH; SP, signal sequence of SP310mut2; LFchimera, chimeric antimicrobial peptide, T, terminator; alr WCFS1, L. plantarum WCFS1 alr gene encoding alanine racemase; repB, a replicon from L. lactis for maintenance in L. lactis; p15A, a replicon from E. coli for maintenance in E. coli.B Restriction mapping analysis. b1) Undigested pAMJ1653 plasmid (Lane 1 and 2, refer to replicates); b2) Double digestion of recombinant pAMJ1653 vector by SapI and SalI. The size of the band of interest after double digestion is 125 bp. C SDS-PAGE analysis of the L. lactis culture supernatant. A protein band with the size of 4.2 kDa (shown by arrow) represents the recombinant cLFchimera peptide. From left to right: lane 1: size marker protein (ladder protein), lane 2: purified cLFchimera from culture supernatant of L. lactis harboring recombinant pAMJ1653 vector and lane 3: culture supernatant of L. lactis harboring self-ligated pAMJ1653 vector with no cLFchimera coding sequence was passed through Ni-NTA agarose column and was loaded on SDS-PAGE as negative control

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us