Fig. 2

a Reduction of ORFV replication by shRNAs. The MDBK cells co- infected with lentivectors expressing shRNA and ORF virus. 72 h after infection, the CPE of ORFV was visualized with a light microscope. ShRNA 1,2 and 3 reduced cytopathic effects of ORFV and MDBK cells had a relatively normal morphology while in the wells infected by ORFV the cell morphology was changed. b Reducing ORFV by recombinant shRNA lentivirus: Extracted total RNA was used for real-time RT-PCR analysis. All the values were displayed in percentages of controls (cells infected with ORFV and those infected with Scrambled vector). The shRNAs 1, 2 and 3 noticeably decreased the expression of viral RNA compared to cells infected with ORFV and scrambled vector (Left chart) and compared to cells infected with ORFV (Right chart). c TCID50 assay: The titration of virus was detected at 48 h pi. The viral yields were measured by the ratio of TCID50 of expressed shRNAs to that of controls (cells infected with ORFV and those infected by Scrambled vector). Error bars show standard deviation of three independent experiments. The shRNAs 1, 2 and 3 reduced virus yield compared to cells infected with ORFV (Left chart) and compared to cells infected with ORFV and scrambled vector (Right chart)