Fig. 4From: A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expressionWestern blot analysis of purified GST-AtRCE2-BIOT and GST-AtRCE2-His6 double-tagged proteins. a The amino acid sequence of the tagging region of GST-AtRCE2-BIOT. b The amino acid sequence of the tagging region of GST-AtRCE2-His6. c, d The double-tagged proteins (GST-AtRCE2-BIOT and GST-AtRCE2-His6) were purified by glutathione affinity beads. The samples were separated by SDS-PAGE, visualized by Coomassie Blue staining and Western blotting with anti-GST, and polyHis-POD antibodies or ExtrAvidin-POD. Affinity purified proteins are marked by asterisks. The molecular weight markers are shown in kDaBack to article page