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Fig. 3 | BMC Biotechnology

Fig. 3

From: A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression

Fig. 3

Western blot analysis of translated Halo-AtMPK9 protein. a Amino acid sequence of tagging region of HaloTag-labelled AtMPK9 vector construct. b Halo-AtMPK9 was purified from total translation mixture with HaloTag affinity beads and AtMPK9 was released from the beads by TEV protease cleavage. The samples were separated by SDS-PAGE and AtMPK9 was detected by immunoblotting with anti-MPK9C antibody. The molecular weight markers are shown in kDa

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BMC Biotechnology

ISSN: 1472-6750

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