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Fig. 2 | BMC Biotechnology

Fig. 2

From: A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression

Fig. 2

Western blot analysis of crude wheat germ protein extract and FLAG-tagged AtMPK9. a Total translation mixture of wheat germ extract (W7240) was separated by TGX Stain-Free gel and imaged by UV light exposure (bottom panel). Following the transfer of proteins to PVDF membrane samples were analysed by Western blotting using anti-polyHis-POD (lane H), anti-GST (lane G), and anti-FLAG (lane F) antibodies. b Amino acid sequence of the tagging region of FLAG-AtMPK9. c Total translation mixture containing FLAG-AtMPK9 was separated by SDS-PAGE and analysed by Western blotting using anti-FLAG antibody. d Total translation mixture containing His6-AtMPK9 was separated by SDS-PAGE and analysed by Western blotting using polyHis-POD antibody. The most abundant non-specific protein signal detected with anti-polyHis-POD is marked by an asterisk. The molecular weight markers are shown in kDa

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BMC Biotechnology

ISSN: 1472-6750

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