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Fig. 1 | BMC Biotechnology

Fig. 1

From: A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression

Fig. 1

IMAC purification and Western blot analysis of His6-AtMPK9 and His12-AtMPK9 proteins. a, b Amino acid sequence of the tagging regions of His6- and His12-labeled AtMPK9 vector constructs. c The in vitro translated His6-AtMPK9 and His12-AtMPK9 were purified with Co-NTA affinity beads, and the total translation mixtures and purified fractions were separated with SDS-PAGE and visualized by Coomassie Blue staining. The purified AtMPK9 proteins are indicated by asterisks. d Serial five-fold dilutions of 1 μl of total translation mixtures were studied by Western blotting using anti-polyHis-POD and anti-MPK9C. The molecular weight markers are shown in kDa

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BMC Biotechnology

ISSN: 1472-6750

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