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Table 2 Disruption efficiency of transforming B. dothidea HTLW03 strain with cassette DNA

From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Gene IDQuantity of DNA (μg)No. of transformantsNo. of correct transformantsTransformation efficiency (No. of transformants/106 protoplasts)GD efficiency (No. of transformants/106 protoplasts)
Bdo_0538152752.7 ± 0.420.5 ± 0.14 bc
103863.8 ± 0.560.6 ± 0.28 bc
1554135.4 ± 0.851.3 ± 0.14 ab
2057165.7 ± 0.491.6 ± 0.14 a
2554175.4 ± 0.421.7 ± 0.21 a
Bdo_02540152212.2 ± 0.560.1 ± 0.14 c
  1. Values are means ± SD from two independent experiments. Values followed with different letters are significantly different (P < 0.05) according to ANOVA and Duncan’s method