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Table 2 Disruption efficiency of transforming B. dothidea HTLW03 strain with cassette DNA

From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Gene ID

Quantity of DNA (μg)

No. of transformants

No. of correct transformants

Transformation efficiency (No. of transformants/106 protoplasts)

GD efficiency (No. of transformants/106 protoplasts)

Bdo_05381

5

27

5

2.7 ± 0.42

0.5 ± 0.14 bc

10

38

6

3.8 ± 0.56

0.6 ± 0.28 bc

15

54

13

5.4 ± 0.85

1.3 ± 0.14 ab

20

57

16

5.7 ± 0.49

1.6 ± 0.14 a

25

54

17

5.4 ± 0.42

1.7 ± 0.21 a

Bdo_02540

15

22

1

2.2 ± 0.56

0.1 ± 0.14 c

  1. Values are means ± SD from two independent experiments. Values followed with different letters are significantly different (P < 0.05) according to ANOVA and Duncan’s method