From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea
Gene ID | Quantity of DNA (μg) | No. of transformants | No. of correct transformants | Transformation efficiency (No. of transformants/106 protoplasts) | GD efficiency (No. of transformants/106 protoplasts) |
---|---|---|---|---|---|
Bdo_05381 | 5 | 27 | 5 | 2.7 ± 0.42 | 0.5 ± 0.14 bc |
10 | 38 | 6 | 3.8 ± 0.56 | 0.6 ± 0.28 bc | |
15 | 54 | 13 | 5.4 ± 0.85 | 1.3 ± 0.14 ab | |
20 | 57 | 16 | 5.7 ± 0.49 | 1.6 ± 0.14 a | |
25 | 54 | 17 | 5.4 ± 0.42 | 1.7 ± 0.21 a | |
Bdo_02540 | 15 | 22 | 1 | 2.2 ± 0.56 | 0.1 ± 0.14 c |