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Table 1 Disruption efficiency of transforming B. dothidea HTLW03 with plasmid or cassette

From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Gene IDDNANo. of transformantsNo. of correct transformantsTransformation efficiency (No. of transformants/106 protoplasts)GD efficiency (No. of transformants/107 protoplasts)
Bdo_05381Cassette3993.9 ± 0.719.0 ± 2.1 a
Plasmid2212.2 ± 0.281.0 ± 1.4 b
  1. Molar mass of 4.34 pmol transforming plasmid or disruption cassette were used. Values are means ± SD from two independent experiments. Values followed with different letters are significantly different according to ANOVA and Duncan’s method (P < 0.05)