Skip to main content

Table 1 Disruption efficiency of transforming B. dothidea HTLW03 with plasmid or cassette

From: An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea

Gene ID

DNA

No. of transformants

No. of correct transformants

Transformation efficiency (No. of transformants/106 protoplasts)

GD efficiency (No. of transformants/107 protoplasts)

Bdo_05381

Cassette

39

9

3.9 ± 0.71

9.0 ± 2.1 a

Plasmid

22

1

2.2 ± 0.28

1.0 ± 1.4 b

  1. Molar mass of 4.34 pmol transforming plasmid or disruption cassette were used. Values are means ± SD from two independent experiments. Values followed with different letters are significantly different according to ANOVA and Duncan’s method (P < 0.05)