Fig. 4From: Optimised production of an anti-fungal antibody in Solanaceae hairy roots to develop new formulations against Candida albicansEvaluation of scFvFc 2G8 stability and proteolytic activity in N. benthamiana or S. lycopersicum HR culture media. Western blot of purified scFvFc 2G8 (150 ng/lane) incubated for different times (0, 4, 24 and 120 h) in MS medium or in PBS (a) or incubated for 4 or 24 h in medium where HR C- of the indicated species were grown (b), with or without the addition of peptidase inhibitors (p.i.), resolved by 10% Tris-Glycine gel in reducing conditions. C+: 150 ng of scFvFc 2G8. Blots were detected with goat anti-human IgG (γ-chain specific) HRP-conjugated antibody. The partial scFvFc 2G8 degradation, mediated by S. lycopersicum proteases, was indicated with solid arrow. c Zymogram of N. benthamiana and S. lycopersicum growth media (GM) concentrated 4 fold and rhizosecretome (RZ) separated on a 10% SDS polyacrylamide gel supplemented with gelatin (0.05%) and stained with colloidal Coomassie blue. M: ColorBurst Electrophoresis Marker (M.W. 8000–220,000 Da)(Sigma-Aldrich)Back to article page