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Fig. 4 | BMC Biotechnology

Fig. 4

From: Optimised production of an anti-fungal antibody in Solanaceae hairy roots to develop new formulations against Candida albicans

Fig. 4

Evaluation of scFvFc 2G8 stability and proteolytic activity in N. benthamiana or S. lycopersicum HR culture media. Western blot of purified scFvFc 2G8 (150 ng/lane) incubated for different times (0, 4, 24 and 120 h) in MS medium or in PBS (a) or incubated for 4 or 24 h in medium where HR C- of the indicated species were grown (b), with or without the addition of peptidase inhibitors (p.i.), resolved by 10% Tris-Glycine gel in reducing conditions. C+: 150 ng of scFvFc 2G8. Blots were detected with goat anti-human IgG (γ-chain specific) HRP-conjugated antibody. The partial scFvFc 2G8 degradation, mediated by S. lycopersicum proteases, was indicated with solid arrow. c Zymogram of N. benthamiana and S. lycopersicum growth media (GM) concentrated 4 fold and rhizosecretome (RZ) separated on a 10% SDS polyacrylamide gel supplemented with gelatin (0.05%) and stained with colloidal Coomassie blue. M: ColorBurst Electrophoresis Marker (M.W. 8000–220,000 Da)(Sigma-Aldrich)

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