Fig. 1

Generation of N. benthamiana and S. lycopersicum HR expressing scFvFc 2G8. a Schematic representation of construct containing the scFv format of the mAb 2G8 fused to the Fc-encoding sequence of the human IgG1 chain or vector used for irrelevant control (pBIc-). SP: signal peptide sequence derived from an embryonic immunoglobulin to direct the recombinant antibody to the secretory pathway [16]; Ω: TMV translational enhancer [17]; 35S prom: Cauliflower Mosaic Virus 35S promoter; Nos ter: Nopaline synthetase terminator sequence. Western blot analysis of TSP (5 μg) extracted from HR expressing scFvFc 2G8 or C- separated on a 10% Tris/glycine gel by SDS-PAGE in reducing (b) or non-reducing (c) conditions. C+: 25 ng of purified scFvFc 2G8. Blots were detected with anti-human IgG1-antibody conjugated to HRP