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Table 1 Mutation profiles induced by CRISPR/Cas9 and number of tragene insertions for each transgenic series

From: Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines

Plant number

Transgene integration

Target gene(s)

Target 1

Target 2

536–2

Chr11

Glyma.16 g090700

∆2-bp, + 1-bp

NA

536–2–13–15

NA

Glyma.16 g090700

No mutations

NA

536–2–13–16

NA

Glyma.16 g090700

∆2-bp

NA

553–6

NA

Glyma.18 g041100

∆7-bp, ∆7-bp

NA

553–6-8

NA

Glyma.18 g041100

∆2-bp

NA

553–6-11

NA

Glyma.18 g041100

∆6-bp

NA

608–1

Chr17

Glyma.16G209100, Glyma.09G159900

∆4-bp, ∆4-bp, + 1-bp

∆4-bp

608–3

Chr06, 09, 16, 18

Glyma.16G209100, Glyma.09G159900

∆3-bp, + 1-bp, TGIa

TGIa

  1. aTGI Transgene integration at CRISPR/Cas9 target site