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Fig. 3 | BMC Biotechnology

Fig. 3

From: Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines

Fig. 3

Screening of mutations and transgene insertions in the transgenic series WPT608–1 and WPT608–3, targeting Glyma.16G209100 and Glyma.09G159900. a) The sequences at the gRNA target sites in the T0 plants compared to the Bert-MN-01 control. Dashes represent a deletion within a sequence, red text represents insertions. Percentages indicate the proportion of reads for each of the mutations in the respective T0 plants. The Glyma.16G209100 sequence is shown in the sense orientation while the Glyma.09G159900 sequence is shown in the antisense orientation. b) The diagram depicts WGS detection of the transgene insertion locus and the variation induced at the insertion site for WPT608–1. Gold represents additions distinct from transgene and reference. The asterisk represents an unresolvable junction due to low sequencing coverage. c) The diagrams depict WGS detection of the transgene insertion loci and the variation induced at the four insertion sites for WPT608–3. Gold represents additions distinct from transgene and reference

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