Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies

Table 1 Primer sequences of the recombinant truncated rBacCPAH6 proteins. The restriction sites and the start codons are underlined with a solid line, in bold the consensus leader sequences

Primers	Nucleotide sequences
rBacCPA 279- EcoRI^b-F	5’-AAGAGAATTC^bTGTATATG^cGGAATCAAAACAAAGGATGT-3’
rBacCPA 279Kozak^a-EcoRI^b-F	5’-AAGAGAATTC^bCAAA^aATG^cGGAATCAAAACAAAGGATGT-3’
rBacCPA 279L21^a-EcoRI^b-F	5’-TGATGAATTC^bAACTCCTAAAAAACCGCCACC^aATG^cGGAATCAAAACAAAGGA-3’
rBacCPA250L21^a-EcoRI^b-F	5’-ATTCGAATTC^bAACTCCTAAAAAACCGCCACC^aATG^cTCAGTTGGAAAGAATG -3’
rBacCPA 363-AgeI^b- R	5’-AAGTACCGGT^bTCCTGAAATCCACTCGTTTATATC-3’
rBacCPA 370-AgeI^b- R	5’-ACTTACCGGT^bTTTTATATTATAAGTTGAATTTCCTGAAATC-3’

ISSN: 1472-6750