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Table 1 Primer sequences of the recombinant truncated rBacCPAH6 proteins. The restriction sites and the start codons are underlined with a solid line, in bold the consensus leader sequences

From: Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies

PrimersNucleotide sequences
rBacCPA 279- EcoRIb-F5’-AAGAGAATTCbTGTATATGcGGAATCAAAACAAAGGATGT-3’
rBacCPA 279Kozaka-EcoRIb-F5’-AAGAGAATTCbCAAAaATGcGGAATCAAAACAAAGGATGT-3’
rBacCPA 279L21a-EcoRIb-F5’-TGATGAATTCbAACTCCTAAAAAACCGCCACCaATGcGGAATCAAAACAAAGGA-3’
rBacCPA250L21a-EcoRIb-F5’-ATTCGAATTCbAACTCCTAAAAAACCGCCACCaATGcTCAGTTGGAAAGAATG -3’
rBacCPA 363-AgeIb- R5’-AAGTACCGGTbTCCTGAAATCCACTCGTTTATATC-3’
rBacCPA 370-AgeIb- R5’-ACTTACCGGTbTTTTATATTATAAGTTGAATTTCCTGAAATC-3’
  1. ain bold= consensus leader sequences
  2. bwith underline= restriction site
  3. cwith underline= start codon