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Fig. 6 | BMC Biotechnology

Fig. 6

From: Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression

Fig. 6

Comparison of the expression of PlyCp41p and PlyCp41pc in plants. Western blot of plant tissue collected 13 days post-infiltration. Neat (O.D. 600 nm = 2.7) and 1:10 (O. D at 600 nm = .27) represent dilutions of Agrobacterium cultures used to infiltrate plants with pGDPVXMCS: PlyCP41pc (CP41pc) or pGDPVXMCS:PlyCP41p mixed in a 1:10 dilution with Agrobacterium containing pGDp19. CP41p (A) designates a plant that was mechanically inoculated from a plant 22 days’ post-infiltration. This sample represents 7 days’ post-infection. rCP41 = 2 μg. M = Precision Plus Kaleidoscope protein standards

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