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Fig. 5 | BMC Biotechnology

Fig. 5

From: Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression

Fig. 5

PlyCP41p protein expression in N. benthamiana plants post-infiltration. Leaf disc samples were collected from infiltrated plants at 2, 3.5, 5, and 10 weeks’ post-infiltration. Western blot analysis was performed using the Penta-His conjugate. N.b., healthy N. benthamiana. M = Precision Plus Kaleidoscope protein standards. This Western blot shows that plant tissue needs to be harvested within 2 weeks’ post-inoculation (w.p.i) to achieve the highest amount of PlyCP41p protein production

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