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Fig. 4 | BMC Biotechnology

Fig. 4

From: Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression

Fig. 4

Purification and quantitation of plant-expressed PlyCP41p. a Plant extracts were processed using Ni-NTA columns under native conditions and analyzed by Western blot of protein using the Penta-His antibody. O, leaf sample extract from virus-infected plants were added to the Ni-NTA column; FT, flow through; W1, Wash 1; W2, Wash 2; E1, Elute 1; E2, Elute 2, E3, Elute 3; E4, Elute 4. rCP41 = 2 μg of Ni-NTA purified PlyCP41 from bacteria. M = Precision Plus Kaleidoscope protein standards. b Quantitation of PlyCP41p in plant extracts. Lanes containing Ni-NTA-purified PlyCP41 from bacteria-concentrations are 0.1 μg, 0.2 μg. 0.5 μg, and 1 μg. The unpurified plant extract (Orig) and the Elution 2 fraction (E2) from Ni-NTA purifications (6/8) and (5/24). The 6/8 purification included protease inhibitor in the extraction buffer. 1 μl of a 100 μl extraction of 4 leaf discs (20 mg plant sample) M = Precision Plus Kaleidoscope protein standards

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BMC Biotechnology

ISSN: 1472-6750

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