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Fig. 4 | BMC Biotechnology

Fig. 4

From: Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

Fig. 4

The fluorescence characterization of GFP purified from N. benthamiana plants. a Polyacrylamide gel was viewed under UV light. Purified GFP was mixed with SDS loading buffer (without reducing agent) and fractionated by electrophoresis through a polyacrylamide gel without prior heat denaturation, then photographed under UV light. Lane1, 2, 3 and 4, twofold serial dilution of purified GFP. b The fluorescence spectrum characterization of purified GFP. The maximum fluorescence excitation peak is at 396 nm; fluorescence emission peak is at 508 nm. Spectra were obtained at a concentration of 0.1 mg/ml and normalized to chromospheres absorption maxima

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